eclipse e400 epifluorescent scope (Nikon)
Structured Review
![D1R antagonist protects against METH-induced cell death. Pretreatment with D1R antagonist, SCH 23390 (SCH [0.05 mg/kg, 0.1 mg/kg, 0.25 mg/kg, 0.5 mg/kg, or 1 mg/kg of body weight]), 30 min before METH (30 mg/kg of body weight, i.p.) abrogated TUNEL-staining in the mouse striata in a dose-dependent fashion. Antagonist alone had no effect. (A) <t>Epifluorescent</t> images of TUNEL-stained mouse striata. Scale bar = 50 µm. (B) Figure shows mean ± SEM percentage of TUNEL-positive staining relative to total neuronal cell counts done previously (data not shown) with neuron-specific nuclear protein NeuN. *P < 0.01 compared with vehicle + saline, !P < 0.005 compared with vehicle + METH 30 mg/kg. No significance is found between regions of the same experimental group.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6203/pmc02886203/pmc02886203__nihms208353f1.jpg)
Eclipse E400 Epifluorescent Scope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eclipse e400 epifluorescent scope/product/Nikon
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Induction of Striatal Pre- and Postsynaptic Damage by Methamphetamine Requires the Dopamine Receptors"
Article Title: Induction of Striatal Pre- and Postsynaptic Damage by Methamphetamine Requires the Dopamine Receptors
Journal:
doi: 10.1002/syn.20185
Figure Legend Snippet: D1R antagonist protects against METH-induced cell death. Pretreatment with D1R antagonist, SCH 23390 (SCH [0.05 mg/kg, 0.1 mg/kg, 0.25 mg/kg, 0.5 mg/kg, or 1 mg/kg of body weight]), 30 min before METH (30 mg/kg of body weight, i.p.) abrogated TUNEL-staining in the mouse striata in a dose-dependent fashion. Antagonist alone had no effect. (A) Epifluorescent images of TUNEL-stained mouse striata. Scale bar = 50 µm. (B) Figure shows mean ± SEM percentage of TUNEL-positive staining relative to total neuronal cell counts done previously (data not shown) with neuron-specific nuclear protein NeuN. *P < 0.01 compared with vehicle + saline, !P < 0.005 compared with vehicle + METH 30 mg/kg. No significance is found between regions of the same experimental group.
Techniques Used: TUNEL Assay, Staining, Saline
Figure Legend Snippet: D2R antagonist protects against METH-induced cell death. Pretreatment with D2R antagonist, raclopride (RAC [0.025 mg/kg, 0.5 mg/kg, or 1 mg/kg of body weight]), 30 min before METH (30 mg/kg of body weight, i.p.) attenuated TUNEL-staining in the mouse striata in a dose-dependent fashion. Antagonist alone had no effect. (A) Epifluorescent images of TUNEL-stained mouse striata. Scale bar = 50 µm. (B) Figure shows mean ± SEM percentage of TUNEL-positive staining relative to total neuronal cell counts done previously (data not shown) with neuron-specific nuclear protein NeuN. *P < 0.01 compared with vehicle + saline, !P < 0.05 compared with vehicle + METH. No significance is found between regions of the same experimental group.
Techniques Used: TUNEL Assay, Staining, Saline
Figure Legend Snippet: D1R and D2R antagonists protect against METH-induced reactive astrocytosis. (A) Figure shows epifluorescent images of GFAP-stained mouse striata. Arrows point to astrocytes stained with anti-GFAP conjugated to Cy3. Scale bar = 10 µm. (B) Pretreatment with D1R (SCH 23390 [0.1 mg/kg of body weight] i.p.) or D2R antagonist, raclopride (RAC [1 mg/kg of body weight] i.p.), 30 min before METH (30 mg/kg of body weight, i.p.) exposure attenuated the induction of reactive astrocysts in the mouse striata. Antagonists alone had no effect. *P < 0.0001 compared with vehicle + saline, !P < 0.0001 compared with vehicle + METH 30 mg/kg. No significance is found between regions. n = 6 per group.
Techniques Used: Staining, Saline